The evolutionary success of higher plants is profoundly linked to their unique cell architecture. The presence of a large central vacuole is fundamental as it enables plants to attain the open and dendritic structure that allows them to explore a large environmental volume with a minimum investment in expensive materials like cellulose and proteins. Besides being low-cost space fillers, vacuoles are the main store for solutes and serve as a hydrostatic skeleton that provides the driving force for cell growth and reversible volume changes. They allow plants to adapt to the fluctuating availability of essential nutrients, to detoxify the cytosol when challenged by harmful molecules, and serve as lysosome-like organelles in which endocytic and autophagic cargo are digested. We are interested in understanding how these multiple functions are co-ordinated and prioritized. Here, we will report on our work regarding redundancy of tonoplast proton-pumps and their interplay with H+-coupled transporters. Moreover, we will share recent insights regarding trafficking of vacuolar proton-pumps and vacuole biogenesis.