By: 
Jan Petrasek, Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic
Host: 
Eva Sundberg
Location: 
Lecture hall A281, Uppsala BioCentre, Ultuna
Date: 
2014-12-18
Time: 
14:00 - 15:00
Searching for mechanisms governing the function and intracellular localization of auxin carriers

A significant proportion of the morphogenic action of auxin has been shown to be regulated by carrier-mediated auxin transport. Auxin carriers transport auxin across the plasma membrane as well as across some endomembranes. Although we know a lot about mechanisms regulation both the function and localization of these carriers, there are still many questions opened that attract our attention. In the talk, I would like to summarize our results from functional testing of auxin influx and efflux carriers in the simplified systems of plant cell lines and discuss their co-operation. In addition, I will also present our recent data on the mechanisms determining the intracellular mobility of auxin carriers and their dynamics within the plasma membrane. Using confocal microscopy, raster image correlation spectroscopy, pharmacological treatments and auxin transport assays, we have uncovered cytoskeleton-dependent mechanism defining both dynamics and function of auxin efflux carriers from PIN family that is not active for AUX1-mediated auxin influx.